Development of Coliphage N4: Ultrastructural Studies
نویسندگان
چکیده
منابع مشابه
Development of coliphage T5: ultrastructural and biochemical studies.
Electron microscopic studies of Escherichia coli infected with bacteriophage T5(+) have revealed that host nuclear material disappeared before 9 min after infection. This disappearance seemed to correspond to the breakdown of host deoxyribonucleic acid (DNA) into acid-soluble fragments. Little or no host DNA thymidine was reincorporated into phage DNA, except in the presence of 5-fluorodeoxyuri...
متن کاملPurification and characterization of the coliphage N4-coded single-stranded DNA binding protein.
We have purified and characterized a single-stranded DNA binding protein (N4 SSB) induced after coliphage N4 infection. It has a monomeric molecular weight of 31,000 and contains 10 tyrosine and 1-2 tryptophan amino acid residues. Its fluorescence spectrum is dominated by the tyrosine residues, and their fluorescence is quenched when the protein binds single-stranded DNA. Fluorescence quenching...
متن کاملUltrastructural studies on the development of human fetal salivary glands.
Parotid and submandibular glands in human fetuses at 16, 18 and 24 weeks of gestation were studied by electron microscopy. The salivary glands of the 16 week fetus were composed of primary ducts and terminal buds. The primary duct was double layered and consisted of inner glycogen-rich ductal cells and outer myoepithelial precursor cells. The terminal bud was a cell cluster which consisted of g...
متن کاملDEVELOPMENT OF A RAPID COLIPHAGE DETECTION ASSAY by
(Abstract) A rapid coliphage detection assay (RCDA), based on the phage-induced release of β-galactosidase from cells of Escherichia coli (Ijzerman, M., J.O. Falkinham III and C. Hagedorn. (1993) [A liquid, colorimetric presence-absence coliphage detection method. J. Virol. Meth. 45:229-234] was modified to reduce the number of steps required to perform the assay, remove the need for specialize...
متن کاملUltrastructural studies on mitochondrial swelling.
Mitochondrial swelling induced by valinomycin, calcium chloride and P(i) was studied after potassium permanganate fixation in suspension. Valinomycin induces a rapid K(+) influx, increase of the matrix space and out-folding of the cristae, with good preservation of the matrix material. This swelling is reversible but the cristae do not completely re-form and have a blebbed appearance. On repeat...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1974
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.13.1.186-196.1974